Molecular Characterization of the Gene Encoding the DNA Gyrase A Subunit of Streptococcus pneumoniae

Author:

Balas Delia1,Fernández-Moreira Esteban1,De La Campa Adela G.1

Affiliation:

1. Unidad de Genética Bacteriana (Consejo Superior de Investigaciones Cientı́ficas), Centro Nacional de Biologı́a Fundamental, Instituto de Salud Carlos III, 28220 Majadahonda, Madrid, Spain

Abstract

ABSTRACT The gene encoding the DNA gyrase A subunit of Streptococcus pneumoniae was cloned and sequenced. The gyrA gene codes for a protein of 822 amino acids homologous to the gyrase A subunit of eubacteria. Translation of the gene in an Escherichia coli expression system revealed a 92-kDa polypeptide. A sequence-directed DNA curvature was identified in the promoter region of gyrA . The bend center was mapped and located between the −35 and −10 regions of the promoter. Primer extension analysis showed that gyrA transcription initiates 6 bp downstream of an extended −10 promoter. The possible implications of the bent DNA region as a regulatory element in the transcription of gyrA are discussed.

Publisher

American Society for Microbiology

Subject

Molecular Biology,Microbiology

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