Identification of Bacillus Spores by Matrix-Assisted Laser Desorption Ionization–Mass Spectrometry-Reference-Cited by-同舟云学术

Identification of Bacillus Spores by Matrix-Assisted Laser Desorption Ionization–Mass Spectrometry

Published:1999-10 Issue:10 Volume:65 Page:4313-4319
ISSN:0099-2240
Container-title:Applied and Environmental Microbiology
language:en
Short-container-title:Appl Environ Microbiol

Author:

Hathout Yetrib¹,Demirev Plamen A.¹,Ho Yen-Peng¹,Bundy Jonathan L.¹,Ryzhov Victor¹,Sapp Lisa¹,Stutler James²,Jackman Joany³,Fenselau Catherine¹

Affiliation:

1. Department of Chemistry and Biochemistry, University of Maryland, College Park, Maryland 207421;

2. GeoCenters, Inc., Fort Detrick, Maryland 217022; and

3. Department of Biochemistry and Molecular Biology, Georgetown University Medical Center, Washington, D.C.3

Abstract

ABSTRACT Unique patterns of biomarkers were reproducibly characterized by matrix-assisted laser desorption ionization (MALDI)–mass spectrometry and were used to distinguish Bacillus species members from one another. Discrimination at the strain level was demonstrated for Bacillus cereus spores. Lipophilic biomarkers were invariant in Bacillus globigii spores produced in three different media and in B. globigii spores stored for more than 30 years. The sensitivity was less than 5,000 cells deposited for analysis. Protein biomarkers were also characterized by MALDI analysis by using spores treated briefly with corona plasma discharge. Protein biomarkers were readily desorbed following this treatment. The effect of corona plasma discharge on the spores was examined.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Link

https://journals.asm.org/doi/pdf/10.1128/AEM.65.10.4313-4319.1999

Reference17 articles.

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