Affiliation:
1. Department of Food Science, University of Wisconsin—Madison, Madison, Wisconsin 53706
Abstract
ABSTRACT
A previously identified insert expressing an endopeptidase from a
Lactobacillus helveticus
CNRZ32 genomic library was characterized. Nucleotide sequence analysis revealed an open reading frame of 1,941 bp encoding a putative protein of 71.2 kDa which contained a zinc-protease motif. Protein homology searches revealed that this enzyme has 40% similarity with endopeptidase O (PepO) from
Lactococcus lactis
P8-2-47. Northern hybridization revealed that
pepO
is monocistronic and is expressed throughout the growth phase. CNRZ32 derivatives lacking PepO activity were constructed via gene replacement. Enzyme assays revealed that the PepO mutant had significantly reduced endopeptidase activity when compared to CNRZ32 with two of the three substrates examined. Growth studies indicated that PepO has no detectable effect on growth rate or acid production by
Lactobacillus helveticus
CNRZ32 in amino acid defined or skim milk medium.
Publisher
American Society for Microbiology
Subject
Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology
Reference34 articles.
1. Basic local alignment search tool.;Altschul S. F.;J. Mol. Biol.,1990
2. Simple and rapid method for isolating large plasmid DNA from lactic streptococci
3. The PROSITE dictionary of sites and patterns in proteins, its current status.;Bairoch A.;Nucleic Acids Res.,1993
4. Accelerated ripening of Gouda cheese. 1. Effect of heat-shocked thermophilic lactobacilli and streptococci on proteolysis and flavor development.;Bartels H. J.;Milchwissenschaft,1987
5. Accelerated ripening of Gouda cheese. 1. Effect of freeze-shocked Lactobacillus helveticus on proteolysis and flavor development.;Bartels H. J.;Milchwissenschaft,1987
Cited by
39 articles.
订阅此论文施引文献
订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献