Determination of Specific Growth Rate by Measurement of Specific Rate of Ribosome Synthesis in Growing and Nongrowing Cultures of Acinetobacter calcoaceticus

Author:

Cutter Matthew R.1,Stroot Peter G.1

Affiliation:

1. Department of Civil and Environmental Engineering, University of South Florida, 4202 East Fowler Ave, ENB118, Tampa, Florida 33620-5350

Abstract

ABSTRACT RT-RiboSyn measures the specific rate of ribosome synthesis in distinct microbial populations by measuring the generation rate of precursor 16S rRNA relative to that of mature 16S rRNA when precursor 16S rRNA processing is inhibited. Good agreement was demonstrated between specific rate of ribosome synthesis and specific growth rate of Acinetobacter calcoaceticus .

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Reference10 articles.

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5. Oerther, D. B, M. C. M. van Loosdrecht, and L. Raskin. 2002. Quantifying the impact of wastewater micronutrient composition on in situ growth activity of Acinetobacter spp. Water Sci. Technol.46(1-2):443-447.

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