Affiliation:
1. Department of Civil and Environmental Engineering, University of Illinois at Urbana-Champaign, Urbana, Illinois 61801,1 and
2. Max-Planck-Institut für Marine Mikrobiologie, D-28359 Bremen, Germany2
Abstract
ABSTRACT
Recently, Cangelosi and Brabant used oligonucleotide probes targeting the precursor 16S rRNA of
Escherichia coli
to demonstrate that the levels of precursor rRNA were more sensitive to changes in growth phase than the levels of total rRNA (G. A. Cangelosi and W. H. Brabant, J. Bacteriol. 179:4457–4463, 1997). In order to measure changes in the levels of precursor rRNA in activated sludge systems, we designed oligonucleotide probes targeting the 3′ region of the precursor 16S rRNA of
Acinetobacter
spp. We used these probes to monitor changes in the level of precursor 16S rRNA during batch growth of
Acinetobacter
spp. in Luria-Bertani (LB) medium, filtered wastewater, and in lab- and full-scale wastewater treatment systems. Consistent with the previous reports for
E. coli
, results obtained with membrane hybridizations and fluorescence in situ hybridizations with
Acinetobacter calcoaceticus
grown in LB medium showed a more substantial and faster increase in precursor 16S rRNA levels compared to the increase in total 16S rRNA levels during exponential growth. Diluting an overnight culture of
A. calcoaceticus
grown in LB medium with filtered wastewater resulted in a pattern of precursor 16S rRNA levels that appeared to follow diauxic growth. In addition, fluorescence in situ hybridizations with oligonucleotide probes targeting total 16S rRNA and precursor 16S rRNA showed that individual cells of
A. calcoaceticus
expressed highly variable levels of precursor 16S rRNA when adapting from LB medium to filtered sewage. Precursor 16S rRNA levels of
Acinetobacter
spp. transiently increased when activated sludge was mixed with influent wastewater in lab- and full-scale wastewater treatment systems. These results suggest that
Acinetobacter
spp. experience a change in growth activity within wastewater treatment systems.
Publisher
American Society for Microbiology
Subject
Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology
Cited by
66 articles.
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