Structural Visualization of Septum Formation in Staphylococcus warneri Using Atomic Force Microscopy

Author:

Su Hai-Nan1,Li Kang12,Zhao Long-Sheng1,Yuan Xiao-Xue1,Zhang Meng-Yao1,Liu Si-Min1,Chen Xiu-Lan1,Liu Lu-Ning34ORCID,Zhang Yu-Zhong132

Affiliation:

1. State Key Laboratory of Microbial Technology, Marine Biotechnology Research Center, Shandong University, Qingdao, China

2. Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao, China

3. College of Marine Life Sciences and Frontiers Science Center for Deep Ocean Multispheres and Earth System, Ocean University of China, Qingdao, China

4. Institute of Systems, Molecular and Integrative Biology, University of Liverpool, Liverpool, United Kingdom

Abstract

This work shows that the septum of Staphylococcus warneri is composed of two layers and that the peptidoglycan on the inner surface of the double-layered septum is organized into concentric rings. Moreover, new cell cycles of S. warneri can be initiated before the previous cell cycle is complete. This work advances our knowledge about a basic structure of bacterial cell and provides information on the double-layered structure of the septum for bacteria that divide with the “popping” mechanism.

Funder

Young Scholars Program of Shandong University

National Key Research and Development Program of China

Program of Shandong Taishan Scholars

Royal Society

Natural Science Foundation of Shandong Province

National Natural Science Foundation of China

UKRI | Biotechnology and Biological Sciences Research Council

Publisher

American Society for Microbiology

Subject

Molecular Biology,Microbiology

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