High-Level Expression and Secretion of Methyl Parathion Hydrolase in Bacillus subtilis WB800-Reference-Cited by-同舟云学术

High-Level Expression and Secretion of Methyl Parathion Hydrolase in Bacillus subtilis WB800

Published:2005-07 Issue:7 Volume:71 Page:4101-4103
ISSN:0099-2240
Container-title:Applied and Environmental Microbiology
language:en
Short-container-title:Appl Environ Microbiol

Author:

Zhang Xiao-Zhou¹,Cui Zhong-Li¹,Hong Qing¹,Li Shun-Peng¹

Affiliation:

1. Department of Microbiology, Key Laboratory for Microbiological Engineering of Agricultural Environment of Ministry of Agriculture, Nanjing Agricultural University, 6 Tongwei Road, Nanjing, Jiangsu 210095, People's Republic of China

Abstract

ABSTRACT The methyl parathion hydrolase (MPH)-encoding gene mpd was placed under the control of the P43 promoter and Bacillus subtilis nprB signal peptide-encoding sequence. High-level expression and secretion of mature, authentic, and stable MPH were achieved using the protease-deficient strain B. subtilis WB800 as the host.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Link

https://journals.asm.org/doi/pdf/10.1128/AEM.71.7.4101-4103.2005

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