Affiliation:
1. Department of Medical Microbiology, University of Sheffield Virus Research Laboratories, Lodge Moor Hospital, and Centre for Human Genetics, United Sheffield Hospitals, Sheffield, England
Abstract
Skin fibroblast cultures were established from eight individuals. These cell cultures, together with WI-38 cells, were examined for susceptibility to transformation by SV40 virus. Four transformation-susceptible cell lines (TS), established from patients with Down's syndrome, were found to be three to four times more susceptible to transformation than transformation-resistant cell lines (TR) from normal individuals. TR and TS cell lines were compared for their susceptibility to induction of SV40 T antigen. For dividing cells T antigen was detected in a higher percentage of TS cells than TR cells. For nondividing cells, the reverse was found; T antigen was detected in 10-fold more cells of the TR lines than in cells of the TS lines. Similar results were obtained after infection of cells with CELO virus. Titration of vaccinia virus and influenza virus A2/Scotland/49/57 indicated that TR and TS cells were equally sensitive to the former virus, but TR cells were three to five times more sensitive to influenza virus A2/Scotland/49/57 than were TS cells.
Publisher
American Society for Microbiology
Subject
Virology,Insect Science,Immunology,Microbiology
Reference18 articles.
1. SV40 T antigen induction and transform--ation in hunian fibroblast cell strains;Aaronson S. A.;Virology.,1968
2. The oncogenic DNA viruses: a review of in vitro transformnation studies. Annu;Black P. H.;Rev. Microbiol.,1968
3. Epidemiology of human leukemia: recent observations;Fraumeni J. E.;J. Nat. Cancer Inst.,1967
4. Huebner R. J. 1967. Adenovirus-directed tumor and T antigens. It M. Pollard (ed.) Perspectives in virology vol. 5. Academ-lic Press Inc. New York.
5. Culture of human fibroblasts for chrontiosome investigations;Hyman J. M.;J. Med. Lab. Technol.,1968
Cited by
32 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献