Rehosting of Bacterial Chaperones for High-Quality Protein Production-Reference-Cited by-同舟云学术

Rehosting of Bacterial Chaperones for High-Quality Protein Production

Published:2009-12-15 Issue:24 Volume:75 Page:7850-7854
ISSN:0099-2240
Container-title:Applied and Environmental Microbiology
language:en
Short-container-title:Appl Environ Microbiol

Author:

Martínez-Alonso Mónica¹²,Toledo-Rubio Verónica¹²,Noad Rob³,Unzueta Ugutz¹²,Ferrer-Miralles Neus¹²,Roy Polly³,Villaverde Antonio¹²

Affiliation:

1. Institute for Biotechnology and Biomedicine and Department of Genetics and Microbiology, Universitat Autònoma de Barcelona, Bellaterra, 08193 Barcelona, Spain

2. CIBER de Bioingeniería, Biomateriales y Nanomedicina (CIBER-BBN), Bellaterra, 08193 Barcelona, Spain

3. Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, Keppel Street, London WC1E 7HT, United Kingdom

Abstract

ABSTRACT Coproduction of DnaK/DnaJ in Escherichia coli enhances solubility but promotes proteolytic degradation of their substrates, minimizing the yield of unstable polypeptides. Higher eukaryotes have orthologs of DnaK/DnaJ but lack the linked bacterial proteolytic system. By coexpression of DnaK and DnaJ in insect cells with inherently misfolding-prone recombinant proteins, we demonstrate simultaneous improvement of soluble protein yield and quality and proteolytic stability. Thus, undesired side effects of bacterial folding modulators can be avoided by appropriate rehosting in heterologous cell expression systems.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Link

https://journals.asm.org/doi/pdf/10.1128/AEM.01532-09

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