Exploiting cyclodextrins as artificial chaperones to enhance enzyme protection through supramolecular engineering

Author:

Foroutan Kalourazi Ali12ORCID,Nazemi Seyed Amirabbas1ORCID,Unniram Parambil Ajmal Roshan13ORCID,Muñoz-Tafalla Ruben45ORCID,Vidal Paula6,Shahangian S. Shirin2,Guallar Victor47ORCID,Ferrer Manuel6ORCID,Shahgaldian Patrick13ORCID

Affiliation:

1. School of Life Science, University of Applied Sciences and Arts Northwestern Switzerland, Hofackerstrasse 30, Muttenz CH-4132, Switzerland

2. Department of Biology, Faculty of Sciences, University of Guilan, Rasht, Iran

3. Swiss Nanoscience Institute, Klingelbergstrasse 82, Basel CH-4056, Switzerland

4. Barcelona Supercomputing Center (BSC), 08034 Barcelona, Spain

5. Faculty of Pharmacy and Food Science, Universitat de Barcelona (UB), 08007 Barcelona, Spain

6. Instituto de Catalisis y Petroleoquimica (ICP), CSIC, 28049 Madrid, Spain

7. Institution for Research and Advanced Studies (ICREA), 08010 Barcelona, Spain

Abstract

Protecting a surface-immobilised enzyme with an enzyme-thin organosilica layer produced using a protein chaperone building block allows drastic improvement in the enzyme's thermal stability.

Funder

H2020 Societal Challenges

Ministerio de Ciencia e Innovación

Agencia Estatal de Investigación

European Regional Development Fund

Publisher

Royal Society of Chemistry (RSC)

Cited by 1 articles. 订阅此论文施引文献 订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献

1. Efficient and easible biocatalysts: Strategies for enzyme improvement. A review;International Journal of Biological Macromolecules;2024-09

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