Simplified Testing Method for Direct Detection of Carbapenemase-Producing Organisms from Positive Blood Cultures Using the NG-Test Carba 5 Assay-Reference-Cited by-同舟云学术

Simplified Testing Method for Direct Detection of Carbapenemase-Producing Organisms from Positive Blood Cultures Using the NG-Test Carba 5 Assay

Published:2019-07 Issue:7 Volume:63 Page:
ISSN:0066-4804
Container-title:Antimicrobial Agents and Chemotherapy
language:en
Short-container-title:Antimicrob Agents Chemother

Author:

Giordano Liliana¹,Fiori Barbara¹²³,D’Inzeo Tiziana¹²,Parisi Gabriella⁴,Liotti Flora Marzia¹,Menchinelli Giulia¹,De Angelis Giulia¹,De Maio Flavio¹,Luzzaro Francesco⁵,Sanguinetti Maurizio¹²^ORCID,Posteraro Brunella⁶⁷,Spanu Teresa¹²

Affiliation:

1. Istituto di Microbiologia, Università Cattolica del Sacro Cuore, Rome, Italy

2. Dipartimento di Scienze di Laboratorio e Infettivologiche, Fondazione Policlinico Universitario A. Gemelli IRCCS, Rome, Italy

3. Scuola Provinciale Superiore di Sanità Claudiana, Bolzano, Italy

4. Azienda Ospedaliera San Camillo-Forlanini, Rome, Italy

5. Azienda Ospedaliera A. Manzoni, Lecco, Italy

6. Istituto di Patologia Medica e Semeiotica Medica, Università Cattolica del Sacro Cuore, Rome, Italy

7. Dipartimento di Scienze Gastroenterologiche, Endocrino-Metaboliche e Nefro-Urologiche, Fondazione Policlinico Universitario A. Gemelli IRCCS, Rome, Italy

Abstract

We directly tested 484 organisms from clinical ( n = 310) and simulated ( n = 174) positive blood cultures using the NG-Test Carba 5 assay for carbapenemase-producing Enterobacterales detection. The assay identified all but 4 of the KPC (170/171), OXA-48-like (22/22), VIM (19/21), and NDM (14/15) producers with no false positives.

Funder

Università Cattolica del S. Cuore

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Pharmacology (medical),Pharmacology

Link

https://journals.asm.org/doi/pdf/10.1128/AAC.00550-19

Reference10 articles.

1. Carbapenemase-Producing Enterobacteriaceae

2. The Epidemiology of Carbapenem-Resistant Enterobacteriaceae: The Impact and Evolution of a Global Menace

3. Multicenter Clinical and Molecular Epidemiological Analysis of Bacteremia Due to Carbapenem-Resistant Enterobacteriaceae (CRE) in the CRE Epicenter of the United States