Affiliation:
1. Istituto Sperimentale Colture Foraggere, 200075 Lodi,1 and
2. Dipartimento di Biologia Animale e Genetica, University of Florence, 50125 Florence,2 Italy
Abstract
ABSTRACT
We analyzed the genetic diversity of 531
Sinorhizobium meliloti
strains isolated from nodules of
Medicago sativa
cultivars in two different Italian soils during 4 years of plant growth. The isolates were analyzed for DNA polymorphism with the random amplified polymorphic DNA method. The populations showed a high level of genetic polymorphism distributed throughout all the isolates, with 440 different haplotypes. Analysis of molecular variance allowed us to relate the genetic structure of the symbiotic population to various factors, including soil type, alfalfa cultivar, individual plants within a cultivar, and time. Some of these factors significantly affected the genetic structure of the population, and their relative influence changed with time. At the beginning of the experiment, the soil of origin and, even more, the cultivar significantly influenced the distribution of genetic variability of
S. meliloti
. After 3 years, the rhizobium population was altered; it showed a genetic structure based mainly on differences among plants, while the effects of soil and cultivar were not significant.
Publisher
American Society for Microbiology
Subject
Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology
Cited by
106 articles.
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