Affiliation:
1. Delft Diagnostic Laboratory, Delft,1 and
2. Department of Medical Microbiology, Free University of Amsterdam, Amsterdam,2 The Netherlands, and
3. Laboratoire de Bactériologie, Hôpital Pellegrin and University Victor Ségalen Bordeaux 2, Bordeaux, France3
Abstract
ABSTRACT
A PCR-based reverse hybridization system (research prototype kit INNO-LiPA for
H. pylori
resistance) was developed and evaluated for simultaneous detection of 23S ribosomal DNA point mutations, associated with macrolide resistance in
Helicobacter pylori
. Fifty-seven
H. pylori
strains (51 natural, 6 laboratory-derived artificial, 52 resistant, and 5 susceptible strains) were tested by PCR-LiPA (detecting mutations A2115→G, G2141→A, A2142→G, A2142→C, A2143→G, A2143→C, and A2143→T), DNA sequencing, restriction fragment length polymorphism, and/or hybridization to oligonucleotide probes. Results were highly concordant, but PCR-LiPA appears to be more sensitive for the simultaneous detection of multiple mutants.
Publisher
American Society for Microbiology
Subject
Infectious Diseases,Pharmacology (medical),Pharmacology
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