Affiliation:
1. Milk and Food Research, Division of Environmental Engineering and Food Protection, Robert A. Taft Sanitary Engineering Center, U.S. Public Health Service, Cincinnati, Ohio
Abstract
Johnson
, H. M. (Robert A. Taft Sanitary Engineering Center, Cincinnati, Ohio), K.
Brenner, R. Angelotti, and H. E. Hall
. Serological studies of types A, B, and E botulinal toxins by passive hemagglutination and bentonite flocculation. J. Bacteriol.
91:
967–974. 1966.—Formalinized sheep red blood cells (SRBC), sensitized with types A, B, and E botulinal toxoids and toxins by bis-diazotized benzidine (BDB), were tested against A, B, and E antitoxins prepared in horses and rabbits. Type B antitoxin cross-reacted with A toxoid SRBC, but the reciprocal cross-reaction was not observed. E toxin SRBC were specifically agglutinated by E antitoxin. Flocculation of antigen-sensitized bentonite particles was less sensitive in titration of antitoxin than hemagglutination. Also, reciprocal cross-reactions were observed between types A and B antitoxins. Cross-reactions in both serological tests were eliminated by titration of antitoxins in the presence of the heterologous antigens, with no inhibitory effect on the homologous antitoxins. Generally, equine antitoxins were less suitable for agglutinations, especially of antigen-sensitized bentonite particles. Types A, B, and E antitoxins were specifically inhibited by 43, 39, and 245 mouse
ld
50
of their respective homologous toxins in the hemagglutination-inhibition test. A, B, and E antitoxins were specifically inhibited by 500, 950, and 1,500 mouse
ld
50
of their respective homologous toxins in bentonite flocculation inhibitions. Formalinized SRBC sensitized with rabbit types A and B antitoxins by BDB were respectively clumped by as little as 0.75 to 1.3 mouse
ld
50
of A toxin and 2.3
ld
50
of B toxin, whereas bentonite particles sensitized by the same antitoxins were specifically clumped by 150
ld
50
of A toxin and 630
ld
50
of B toxin. E antitoxin sensitization of SRBC or bentonite particles was not successful. Evidence is presented that indicates that the serological procedures are applicable to the detection of botulinal toxins in food.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
Reference18 articles.
1. Study of toxins of Clostridium botulinum. III. Relation of autolysis to toxin production;BOROFF D. A.;J. Bacteriol.,1955
2. Preparation and assay of the international standards for Clostridium botulinum types A, B, C, D, and E antitoxins;BOWMER E. J.;Bull. World Health Organ.,1963
3. Desoxyribonucleic acid (DNA)-bentonite flocculation test for Iupus erythematosus;BOZICEVICH J., J.;Proc. Soc. Exptl. Biol. Med.,1960
4. The bentonite flocculation test for detection of plant viruses and titration of antibody;BOZICEVICH J., H.;Proc. Soc. Exptl. Biol. Med.,1963
5. Hemagglutination studies with formalinized erythrocytes. Effect of bisdiazotized benzidine and tannic acid treatment on sensitization by soluble antigen;BUTLER W. T.;J. Immunol.,1963
Cited by
50 articles.
订阅此论文施引文献
订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献