Affiliation:
1. Food Research Institute, University of Wisconsin, Madison 53706.
Abstract
The kinetics of toxic shock syndrome toxin 1 (TSST-1) production by Staphylococcus aureus was studied in a fermentor in which aeration rate, atmospheric composition, pH, and temperature were controlled. The toxin was synthesized at a maximal rate during the exponential phase. High bacterial populations were not necessarily accompanied by high TSST-1 yields. Aerobiosis increased TSST-1 production, but excessive aeration had an adverse effect. Addition of CO2 enhanced TSST-1 yield by increasing toxin production rate and efficiency. Cultures with no pH control made more TSST-1 than those maintained at pH 5.5 to 7.5. Maximum TSST-1 yields were obtained when cultures were supplied with air (20 cm3/min) and CO2 (5 cm3/min) via a sintered glass sparger.
Publisher
American Society for Microbiology
Subject
Infectious Diseases,Immunology,Microbiology,Parasitology
Reference20 articles.
1. Arvidson S. A. Bjorkland R. Eriksson and T. Holme. 1976. Enzymes from Staphylococc us aureus p. 238-250. In A. C. R. Dean D. C. Ellwood C. G. T. Evans and J. Melling (ed.). Continuous culture: application and new fields. Proceedings of the Sixth International Symposium on Continuous Cultures of Microorganisms. Ellis Horwood Chichester England.
2. Bergdoll M. S. and P. M. Schlievert. 1984. Toxic shock syndrome toxin. Lancet ii:69.
3. The production of staphylococcal toxin;Burnet F. M.;J. Pathol. Bacteriol.,1930
4. A CO,-enhanced hemolytic activity of Staphylococcius aiur-eius associated with toxic shock syndrome: inhibition by agar;Carlson E. C.;J. Infect. Dis.,1986
5. Staphylococcal enterotoxin B and nuclease production under controlled oxygen conditions;Carpenter D. F.;Appl. Microbiol.,1974
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