Robust Real-Time Reverse Transcription-PCR for Detection of Foot-and-Mouth Disease Virus Neutralizing Carryover Contamination-Reference-Cited by-同舟云学术

Robust Real-Time Reverse Transcription-PCR for Detection of Foot-and-Mouth Disease Virus Neutralizing Carryover Contamination

Published:2016-01 Issue:1 Volume:54 Page:216-219
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Hwang Ji-Hyeon¹,Shin Yong-Keol²,Park So-Yeon²,Kim Jeesoo¹,Kim Su-Mi¹,Kim Byounghan¹,Park Jong-Hyeon¹,Lee Jong-Soo³,Lee Kwang-Nyeong¹

Affiliation:

1. Foot and Mouth Disease Division, Animal and Plant Quarantine Agency, Anyang, Republic of Korea

2. Enzynomics, Inc., Daejeon, Republic of Korea

3. Veterinary College, Chungnam National University, Daejeon, Republic of Korea

Abstract

ABSTRACT During an outbreak of foot-and-mouth disease (FMD), real-time reverse transcription-PCR (rRT-PCR) is the most commonly used diagnostic method to detect viral RNA. However, while this assay is often conducted during the outbreak period, there is an inevitable risk of carryover contamination. This study shows that the carryover contamination can be prevented by the use of target-specific restriction endonuclease in that assay.

Funder

Animal and plant quarantine agency

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.01944-15

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