Low‐copy transgene detection using nested digital polymerase chain reaction for gene‐doping control
Author:
Affiliation:
1. Genetic Analysis Department Laboratory of Racing Chemistry Utsunomiya Tochigi Japan
2. Equine Genetics Research Centre Racing Australia Scone New South Wales Australia
3. Equine Department Japan Racing Association Minato Tokyo Japan
Funder
Japan Racing Association
Publisher
Wiley
Subject
Spectroscopy,Pharmaceutical Science,Environmental Chemistry,Analytical Chemistry
Link
https://onlinelibrary.wiley.com/doi/pdf/10.1002/dta.3173
Reference26 articles.
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2. Establishment of two quantitative nested qPCR assays targeting the human EPO transgene;Neuberger EW;Gene Ther,2016
3. Improved detection of transgene and nonviral vectors in blood;Baoutina A;Hum Gene Ther Methods,2013
4. Transgene traceability in transgenic mice: a bioanalytical approach for potential gene‐doping analysis;Bogani P;Bioanalysis,2011
5. Loop‐mediated isothermal amplification (LAMP) as an alternative to PCR: a rapid on‐site detection of gene doping;Salamin O;Drug Tes Ana,2017
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