Method for recovering viruses from river water solids

Author:

Berg G,Dahling D R

Abstract

Small numbers of virions (poliovirus 1) that had been adsorbed to river water solids were eluted by mixing the solids for 30 min with a 10% solution of beef extract that contained sufficient Na2HPO4 to bring the molarity of the salt to 0.05 and sufficient citric acid to bring the pH to 7. The virions were recovered by inoculating the beef extract onto cell cultures. With this method, 39 to 63% of the poliovirions that had been adsorbed onto the river water solids were recovered.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Reference8 articles.

1. Berg G. 1973. Discussion of: Concentration of viruses from seawater p. 117-118. In S. H. Jenkins (ed.) Advances in water pollution research. Pergamon Press Ltd. Oxford England.

2. Reasmessent of the virus problem in sewage and in surface and renovated waters;Berg G.;Prog. Water Technol.,1973

3. Recovery of small quantities of viruses from clean waters on cellulose nitrate membrane filters;Berg G.;Appl. Microbiol.,1971

4. Berg G. and T. G. Metcalf. 1978. Indicators of viruses in water p. 267-296. In G. Berg (ed.) Indicators of viruses in water and food. Ann Arbor Science Publishers Inc. Ann Arbor Mich.

5. BGM, a continuous cell line more sensitive tham primary rhesus and African green kidney cells for the recovery of viruses from water;Dahling D. R.;Health Lab. Sci.,1974

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