A Double-Selective Tissue Culture System for Isolation of Wild-Type Poliovirus from Sewage Applied in a Long-Term Environmental Surveillance-Reference-Cited by-同舟云学术

A Double-Selective Tissue Culture System for Isolation of Wild-Type Poliovirus from Sewage Applied in a Long-Term Environmental Surveillance

Published:1999-04 Issue:4 Volume:65 Page:1794-1797
ISSN:0099-2240
Container-title:Applied and Environmental Microbiology
language:en
Short-container-title:Appl Environ Microbiol

Author:

Manor Y.¹,Handsher R.¹,Halmut T.¹,Neuman M.¹,Abramovitz B.¹,Mates A.²,Mendelson E.¹

Affiliation:

1. Central Virology Laboratory, Public Health Laboratories, Chaim Sheba Medical Center, Tel-Hashomer,1 and

2. Department of Laboratories, Ministry of Health, Jerusalem,2 Israel

Abstract

ABSTRACT We describe a simple, cost-efficient, double-selective method for isolation of wild-type poliovirus from sewage samples containing vaccine polioviruses and other enteroviruses, with a detection limit of 18 to 50 PFU per 1 to 2 liters of sewage. By this method we were able to process 1,700 sewage samples collected between 1991 and 1996, from which 10,472 plaques were isolated, 41 of them being identified as wild-type polioviruses.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Link

https://journals.asm.org/doi/pdf/10.1128/AEM.65.4.1794-1797.1999

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