Affiliation:
1. Laboratoire de Génétique Microbienne, Institut National de la Recherche Agronomique, Jouy en Josas, France.
Abstract
An internal fragment of the recA gene from eight gram-positive organisms has been amplified by using degenerate primers in a polymerase chain reaction. The internal 348- or 360-bp recA DNA segments from Bacillus subtilis, Clostridium acetobutylicum, Lactobacillus bulgaricus, Lactobacillus helveticus, Leuconostoc mesanteroides, Listeria monocytogenes, Staphylococcus aureus, and Streptococcus salivarus subsp. thermophilus were amplified, cloned, and sequenced. The G + C contents of the DNA from these species range from 28 to 52%. The sequences of the bacterial recA genes show strong relatedness. This method is particularly useful for the recovery of the recA genes of gram-positive bacteria and avoids the difficulties of using a genetic complementation test for cloning.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
Reference15 articles.
1. Multiple sequence alignment with hierarchical clustering;Corp;Nucleic Acids Res.,1988
2. Novel structure of the recA locus of Mycobacterium tuberculosis implies processing of the gene product;Davis E. O.;J. Bacteriol.,1991
3. Duwat P. S. D. Ehrlich and A. Gruss. PCR cloning and sequencing of the L. lactis subsp. lactis recA gene. Appl. Environ. Microbiol. in press.
4. Cloning and DNA sequence of a mycoplasmal recA gene;Dybvig K.;J. Bacteriol.,1992
5. Fidelity of DNA polymerases in DNA amplification;Keohavong X.;Proc. Natl. Acad. Sci. USA,1989
Cited by
40 articles.
订阅此论文施引文献
订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献