Evidence for two recA genes mediating DNA repair in Bacillus megaterium

Abstract

Isolation and subsequent knockout of arecA-homologous gene inBacillus megateriumDSM 319 resulted in a mutant displaying increased sensitivity to mitomycin C. However, this mutant did not exhibit UV hypersensitivity, a finding which eventually led to identification of a second functionalrecAgene. Evidence forrecAduplicates was also obtained for two otherB. megateriumstrains. In agreement with potential DinR boxes located within their promoter regions, expression of both genes (recA1andrecA2) was found to be damage-inducible. Transcription from therecA2promoter was significantly higher than that ofrecA1. Since arecA2knockout could not be achieved, functional complementation studies were performed inEscherichia coli. Heterologous expression in a RecA null mutant resulted in increased survival after UV irradiation and mitomycin C treatment, proving bothrecAgene products to be functional in DNA repair. Thus, there is evidence for an SOS-like pathway inB. megateriumthat differs from that ofBacillus subtilis.