Isolation and Analysis of the Nucleic Acids and Polysaccharides from Clostridium welchii

Abstract

A method previously described for the use of bentonite in the isolation of the nucleic acids from two gram-positive organisms was applied to the isolation of the nucleic acids from two strains of Clostridium welchii . The nucleic acids were separated from polysaccharides by the fractional precipitation of their cetyltrimethyl-ammonium salts from sodium chloride solution, and the base composition of the nucleic acids was determined. One strain of C. welchii investigated (NCTC 10578) was shown to produce considerable quantities of an acidic and also a weakly acidic or neutral polysaccharide; the other strain (ATCC 10543) gave very small quantities of the latter but none of the former polysaccharide. The monosaccharide composition of these polysaccharides was determined and the acidic polysaccharide was shown to resemble dermatan sulfate.