A phenol/chloroform-free method to extract nucleic acids from recalcitrant, woody tropical species for gene expression and sequencing-Reference-Cited by-同舟云学术

A phenol/chloroform-free method to extract nucleic acids from recalcitrant, woody tropical species for gene expression and sequencing

Published:2019-06-05 Issue:1 Volume:15 Page:
ISSN:1746-4811
Container-title:Plant Methods
language:en
Short-container-title:Plant Methods

Author:

Barbier François F.^ORCID,Chabikwa Tinashe G.^ORCID,Ahsan Muhammad U.^ORCID,Cook Stacey E.^ORCID,Powell Rosanna^ORCID,Tanurdzic Milos^ORCID,Beveridge Christine A.^ORCID

Funder

Australian Research Council, the Queensland Government and the Horticulture Innovation Australia Limited

Publisher

Springer Science and Business Media LLC

Subject

Plant Science,Genetics,Biotechnology

Link

http://link.springer.com/content/pdf/10.1186/s13007-019-0447-3.pdf

Reference38 articles.

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2. Kasajima I. Protocol for DNA extraction from any plant species (alkaline PVPP method). 2017 [cited 2018 Jun 13]; Available from: https://www.nature.com/protocolexchange/protocols/5359#/procedure .

3. Berendzen K, Searle I, Ravenscroft D, Koncz C, Batschauer A, Coupland G, et al. A rapid and versatile combined DNA/RNA extraction protocol and its application to the analysis of a novel DNA marker set polymorphic between Arabidopsis thaliana ecotypes Col-0 and Landsberg erecta. Plant Methods. 2005;1:4.

4. Zou Y, Mason MG, Wang Y, Wee E, Turni C, Blackall PJ, et al. Nucleic acid purification from plants, animals and microbes in under 30 seconds. PLoS Biol. 2017;15:e2003916.

5. Schneiderbauer A, Sandermann H, Ernst D. Isolation of functional RNA from plant tissues rich in phenolic compounds. Anal Biochem. 1991;197:91–5.

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