Affiliation:
1. Australian Atomic Energy Commission, Lucas Heights, Sutherland, New South Wales, Australia
Abstract
The kinetics of initiation of germination and inactivation by hydrostatic pressure of phosphate-buffered
Bacillus pumilus
spores is shown to be a consecutive first-order process at 25 C. The effect of increasing pressure at constant temperature was studied, and rate constants were derived by using the criteria of heat resistance, refractility, and stainability. The calculated volume change of activation (Δ
V
‡) was −139 ± 6 cm
3
/mole for loss of heat resistance, −158 ± 8 cm
3
/mole for the loss of refractility, and −153 ± 4 cm
3
/mole for the change in permeability to dilute stains for the pressure range 800 to 1,010 atm at 25 C. It is suggested that the spore exists as a Donnan phase and that pressure triggers germination by influencing the equilibrium.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
Reference10 articles.
1. Base exchange and heat resistance in bacterial spores;Alderton G.;Biochem. Biophys. Res. Commun.,1963
2. Initiation of germination and inactivation of Bacillus pumilus spores by hydrostatic pressure;Clouston J. G.;J. Bacteriol.,1969
3. Frost A. A. and R. G. Pearson. 1961. Kinetics and mechanism 2nd ed. p. 166. John Wiley & Sons Inc. New York.
4. Initiation of germination of bacterial spores by hydrostatic pressure;Gould G. W.;J. Gen. Microbiol.,1970
5. Helifferich F. 1962. Ion exchange p. 158 McGraw-Hill Book Co. Inc. New York.
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