Purification and cDNA Cloning of NADPH-Dependent Aldoketoreductase, Involved in Asymmetric Reduction of Methyl 4-Bromo-3-Oxobutyrate, from Penicillium citrinum IFO4631-Reference-Cited by-同舟云学术

Purification and cDNA Cloning of NADPH-Dependent Aldoketoreductase, Involved in Asymmetric Reduction of Methyl 4-Bromo-3-Oxobutyrate, from Penicillium citrinum IFO4631

Published:2005-02 Issue:2 Volume:71 Page:1101-1104
ISSN:0099-2240
Container-title:Applied and Environmental Microbiology
language:en
Short-container-title:Appl Environ Microbiol

Author:

Asako Hiroyuki¹,Wakita Ryuhei¹,Matsumura Kenji²,Shimizu Masatoshi¹,Sakai Jun³,Itoh Nobuya⁴

Affiliation:

1. Organic Synthesis Research Laboratory, Sumitomo Chemical Co., Ltd.

2. Agricultural Research Laboratory, Sumitomo Chemical Co., Ltd., Takarazuka, Hyogo

3. Genome Science Laboratories, Sumitomo Pharmaceuticals Co., Ltd., Konohana-ku, Osaka

4. Biotechnology Research Center, Toyama Prefectural University, Toyama, Japan

Abstract

ABSTRACT Penicillium citrinum was found to catalyze the reduction of methyl 4-bromo-3-oxobutyrate to methyl ( S )-4-bromo-3-hydroxybutyrate [( S )-BHBM] with high optical purity. From the strain, a cDNA clone encoding a novel NADPH-dependent alkyl 4-halo-3-oxobutyrate reductase (KER) was isolated. Escherichia coli cells overexpressing KER produced ( S )-BHBM in the presence of an NADPH-regeneration system.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Link

https://journals.asm.org/doi/pdf/10.1128/AEM.71.2.1101-1104.2005

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