Identification of Vibrio parahaemolyticus Strains at the Species Level by PCR Targeted to the toxR Gene-Reference-Cited by-同舟云学术

Identification of Vibrio parahaemolyticus Strains at the Species Level by PCR Targeted to the toxR Gene

Published:1999-04 Issue:4 Volume:37 Page:1173-1177
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Kim Yung Bu¹,Okuda Jun¹,Matsumoto Chiho¹,Takahashi Naoki²,Hashimoto Satoru²,Nishibuchi Mitsuaki¹

Affiliation:

1. Center for Southeast Asian Studies, Kyoto University, Yoshida, Sakyo-ku, Kyoto,1 and

2. Kansai Airport Quarantine Station, Tajiri, Sennan, Osaka,2 Japan

Abstract

ABSTRACT The DNA colony hybridization test with the polynucleotide probe for Vibrio parahaemolyticus toxR gene was performed. All 373 strains of V. parahaemolyticus gave positive results, and the strains belonging to four other Vibrio species including Vibrio alginolyticus gave weakly positive results, suggesting that toxR sequence variation may reflect the phylogenetic relationships of Vibrio species. We then established a toxR -targeted PCR protocol for the specific detection of V. parahaemolyticus .

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.37.4.1173-1177.1999

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