Multicenter Trials Need To Use the Same Assay for Hepatitis C Virus Viral Load Determination-Reference-Cited by-同舟云学术

Multicenter Trials Need To Use the Same Assay for Hepatitis C Virus Viral Load Determination

Published:2007-11 Issue:11 Volume:45 Page:3788-3790
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Laperche Syria¹,Bouchardeau Françoise¹,Thibault Vincent²,Pozzetto Bruno³,Vallet Sophie⁴,Rosenberg Arielle R.⁵,Roque-Afonso Anne-Marie⁶,Gassin Michèle⁷,Stoll-Keller Françoise⁸,Trimoulet Pascale⁹,Gault Elyanne¹⁰,Chanzy Bruno¹¹,Mercier Bernard¹²,Branger Michel¹³,Pawlotsky Jean-Michel¹⁴,Henquell Cécile¹⁵,Lunel Françoise¹⁶,Gaudy-Graffin Catherine¹⁷,Alain Sophie¹⁸,Chaix Marie-Laure¹⁹,Duverlie Gilles²⁰,Izopet Jacques²¹,Lefrère Jean-Jacques²²²³

Affiliation:

1. Centre National de Référence pour les hépatites B et C en transfusion, Département des Agents Transmissibles par le Sang, Institut National de la Transfusion Sanguine, Paris

2. Laboratoire de Virologie, Centre Hospitalo-Universitaire Pitié-Salpêtrière, Paris

3. Laboratoire de Virologie-Bactériologie, Centre Hospitalo-Universitaire, Saint-Etienne

4. Laboratoire de Microbiologie, Centre Hospitalo-Universitaire, Brest

5. Université René Descartes, Faculté de Médecine, and AP-HP, Groupe Hospitalier Cochin, Paris

6. Laboratoire de Virologie, Centre Hospitalier Paul Brousse, Villejuif

7. Institut de Biologie, Hôtel Dieu, Nantes

8. Laboratoire de Virologie, Centre Hospitalier, Strasbourg

9. Laboratoire de Virologie, Centre Hospitalo-Universitaire, Bordeaux

10. Laboratoire de Virologie-Bactériologie associé au CNR des hépatites B et C, Centre Hospitalo-Universitaire Avicenne, Bobigny

11. Laboratoire de Virologie, Centre Hospitalier, Annecy

12. Laboratoire de Virologie, Etablissement Français du Sang Bretagne, Brest

13. Laboratoire de Virologie, Centre Hospitalo-Universitaire Bichat, Paris

14. Laboratoire de Virologie-Bactériologie, Centre Hospitalo-Universitaire Henri-Mondor, Créteil

15. Laboratoire de Virologie, Centre Hospitalo-Universitaire, Clermont-Ferrand

16. Laboratoire de Virologie-Bactériologie, Centre Hospitalo-Universitaire, Angers

17. Université François Rabelais, Inserm ERI19, Centre Hospitalier Universitaire, Tours

18. Laboratoire de Virologie-Bactériologie, Centre Hospitalo-Universitaire, Limoges

19. Laboratoire de Virologie, Centre Hospitalo-Universitaire Necker, Paris

20. Laboratoire de Virologie, Centre Hospitalo-Universitaire, Amiens

21. Laboratoire de Virologie, Centre Hospitalo-Universitaire, Toulouse

22. Laboratoire d'Hématologie, Centre Hospitalo-Universitaire, Amiens

23. Département des Agents Transmissibles par le Sang, Institut National de la Transfusion Sanguine, Paris, France

Abstract

ABSTRACT This study, involving 20 laboratories and using currently available assays for hepatitis C virus RNA quantification, demonstrated that differences in viral load values are due not to interlaboratory variations but rather to the nature of the assay itself. This underlines the importance of using the same assay in multicenter studies or when monitoring antiviral therapy.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.00825-07

Reference12 articles.

1. Performance Characteristics of a Quantitative TaqMan Hepatitis C Virus RNA Analyte-Specific Reagent

2. Multilaboratory Comparison of Hepatitis C Virus Viral Load Assays

3. Verification of an Assay for Quantification of Hepatitis C Virus RNA by Use of an Analyte-Specific Reagent and Two Different Extraction Methods

4. French Study Group for the Standardization of Hepatitis C Virus PCR. 1994. Improvement of hepatitis C virus RNA polymerase chain reaction through a multicentre quality control study. J. Virol. Methods49:79-88.

5. Gelderblom, H. C., S. Menting, and M. G. Beld. 2006. Clinical performance of the new rRoche COBAS TaqMan HCV test and High Pure system for extraction, detection and quantitation of HCV RNA in plasma and serum. Antivir. Ther.11:95-103.

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