A DEFINED AGAR MEDIUM FOR GENETIC TRANSFORMATION OF NEISSERIA MENINGITIDIS

Author:

Catlin B. Wesley1,Schloer Gertrude M.1

Affiliation:

1. Department of Microbiology and Immunology, School of Medicine, Marquette University, Milwaukee, Wisconsin

Abstract

Catlin, B. Wesley (Marquette University, Milwaukee, Wis.) and Gertrude M. Schloer . A defined agar medium for genetic transformation of Neisseria meningitidis . J. Bacteriol. 83: 470–474. 1962.—An agar medium was developed for use in quantitative genetic studies of Neisseria meningitidis strain 15. It contains eight inorganic salts, sodium citrate, sodium lactate, arginine, cysteine, glycine, sodium glutamate, and purified agar. Abundant surface growth in the absence of supplemental carbon dioxide was obtained during 50 serial subcultures. A close correspondence was found between numbers of parental type colonies developing on the defined medium and on a complex medium. Cells subcultured serially three or four times on defined agar medium and placed directly into a solution of transforming deoxyribonucleic acid in defined liquid medium were susceptible to transformation without additional supplements. Of the treated population, 0.1 to 0.3% of the cells were transformed to streptomycin resistance.

Publisher

American Society for Microbiology

Subject

Molecular Biology,Microbiology

Cited by 23 articles. 订阅此论文施引文献 订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献

1. A large genomic island allows N eisseria meningitidis to utilize propionic acid, with implications for colonization of the human nasopharynx;Molecular Microbiology;2014-06-27

2. Biologic Activities of the TolC-Like Protein of Neisseria meningitidis as Assessed by Functional Complementation in Escherichia coli;Antimicrobial Agents and Chemotherapy;2010-01

3. FATE OF EXOGENOUS DNA IN NEISSERIA MENINGITIDIS;Acta Pathologica Microbiologica Scandinavica Section B Microbiology and Immunology;2009-08-15

4. UTILIZATION OF THYMINE, THYMIDINE AND TMP BY NEISSERIA MENINGITIDIS;Acta Pathologica Microbiologica Scandinavica Section B Microbiology and Immunology;2009-08-15

5. ELIMINATION OF GENETIC ELEMENTS GOVERNING COMPETENCE IN TRANSFORMATION OF NEISSERIA MENINGITIDIS BY TREATMENT WITH ETHIDIUM BROMIDE AND ACRIFLAVIN;Acta Pathologica Microbiologica Scandinavica Section B Microbiology and Immunology;2009-08-15

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3