Reduced In Vitro Susceptibility of Streptococcus pyogenes to β-Lactam Antibiotics Associated with Mutations in the pbp2x Gene Is Geographically Widespread-Reference-Cited by-同舟云学术

Reduced In Vitro Susceptibility of Streptococcus pyogenes to β-Lactam Antibiotics Associated with Mutations in the pbp2x Gene Is Geographically Widespread

Published:2020-03-25 Issue:4 Volume:58 Page:
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Musser James M.¹²³,Beres Stephen B.¹²,Zhu Luchang¹²,Olsen Randall J.¹²³,Vuopio Jaana⁴⁵,Hyyryläinen Hanne-Leena⁶,Gröndahl-Yli-Hannuksela Kirsi⁴,Kristinsson Karl G.⁷⁸,Darenberg Jessica⁹,Henriques-Normark Birgitta¹⁰¹¹,Hoffmann Steen¹²,Caugant Dominque A.¹³,Smith Andrew J.¹⁴¹⁵,Lindsay Diane S. J.¹⁵,Boragine David M.¹⁶,Palzkill Timothy¹⁷^ORCID

Affiliation:

1. Center for Molecular and Translational Human Infectious Diseases Research, Houston Methodist Research Institute and Houston Methodist Hospital, Houston, Texas, USA

2. Clinical Microbiology Laboratory, Department of Pathology and Genomic Medicine, Houston Methodist Research Institute and Houston Methodist Hospital, Houston, Texas, USA

3. Department of Pathology and Laboratory Medicine, Weill Cornell Medical College, New York, New York, USA

4. Institute of Biomedicine, University of Turku, Turku, Finland

5. Turku University Hospital, Department of Clinical Microbiology, Turku, Finland

6. Department of Health Security, Finnish Institute for Health and Welfare, Helsinki, Finland

7. Department of Clinical Microbiology, Landspitali University Hospital, Reykjavik, Iceland

8. Faculty of Medicine, School of Health Sciences, University of Iceland, Reykjavik, Iceland

9. Public Health Agency of Sweden, Solna, Sweden

10. Department of Microbiology, Tumor and Cell Biology, Karolinska Institutet, Stockholm, Sweden

11. Department of Laboratory Medicine, Division of Clinical Microbiology, Karolinska University Hospital, Stockholm, Sweden

12. Neisseria and Streptococcus Reference Laboratory, Bacteria, Parasites & Fungi, Infectious Disease Preparedness, Statens Serum Institut, Copenhagen, Denmark

13. Division for Infection Control and Environmental Health, Norwegian Institute of Public Health, Oslo, Norway

14. College of Medical, Veterinary and Life Sciences, Glasgow Dental Hospital and School, University of Glasgow, Glasgow, Scotland

15. Scottish Microbiology Reference Laboratory, Glasgow, Scotland

16. Department of Biochemistry and Molecular Biology, Baylor College of Medicine, Houston, Texas, USA

17. Department of Pharmacology and Chemical Biology, Baylor College of Medicine, Houston, Texas, USA

Abstract

Recently, two related Streptococcus pyogenes strains with reduced susceptibility to ampicillin, amoxicillin, and cefotaxime, antibiotics commonly used to treat S. pyogenes infections, were reported. The two strains had the same nonsynonymous (amino acid-substituting) mutation in the pbp2x gene, encoding penicillin-binding protein 2X (PBP2X). This concerning report led us to investigate our library of 7,025 genome sequences of type emm1 , emm28 , and emm89 S. pyogenes clinical strains recovered from intercontinental sources for mutations in pbp2x .

Funder

Division of Extramural Research, National Institute of Allergy and Infectious Diseases

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.01993-19

Reference52 articles.

1. Why Have Group A Streptococci Remained Susceptible to Penicillin? Report on a Symposium

2. Group A Streptococcal Diseases and Their Global Burden

3. Streptococcus pyogenes pbp2x Mutation Confers Reduced Susceptibility to β-Lactam Antibiotics