Affiliation:
1. Departments of Dairy Science and Microbiology, University of Illinois, Urbana, Illinois 61801
Abstract
S organism ferments ethanol to acetate and H
2
but grows poorly on ethanol unless the partial pressure of H
2
is kept low, as when it is grown in combination with an H
2
-utilizing methanogenic bacterium. The present study shows that S organism contains an alcohol dehydrogenase and a formate dehydrogenase, both of which require nicotinamide adenine dinucleotide (NAD) for activity. Hydrogen is evolved from NADH generated by these activities via a ferredoxin-dependent oxidation of NADH to NAD and H
2
. NADH:NADP oxido-reductase activity was also demonstrated. The relationship of these activities to the growth of S organism is discussed.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
Reference22 articles.
1. Electron transport in Peptostreptococcus elsdenii;Baldwin R. L.;Biochim. Biophys. Acta,1964
2. Studies upon the methane fermentation. IV. The isolation and culture of Methanobacillus omelianskii;Barker H. A.;Antonie Van Leeuwenhoek J. Microbiol. Serol.,1940
3. Studies on the methane fermentation. V. Biochemical activities of Methanobacterium omelianskii;Barker H. A.;J. Biol. Chem.,1941
4. Anaerobic formate oxidation: a ferredoxin-dependent reaction;Brill W. J.;Science,1964
5. Hydrogen-oxidizing methane bacteria. I. Cultivation and methanogenesis;Bryant M. P.;J. Bacteriol.,1968
Cited by
49 articles.
订阅此论文施引文献
订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献