Affiliation:
1. Departments of Microbiology and Dairy Science, University of Illinois, Urbana, Illinois 61801
Abstract
A method for the mass culture of hydrogen-oxidizing methane bacteria has been developed; yields of 50 to 60 g (wet weight) of cells per 12 to 14 liters of culture medium were obtained. The methanogenic organism from the culture of
Methanobacillus omelianskii
was grown in a complex medium which was aerated with a gas mixture of hydrogen and carbon dioxide. Extracts prepared from hydrogen-grown cells formed methane from methyl cobalamin, 5-methyl tetrahydrofolate, serine, pyruvate, and carbon dioxide; these substrates have been shown to be precursors of methane in extracts of the ethyl alcohol-grown culture of
M. omelianskii
.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
Cited by
107 articles.
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