Plasmid Involvement in Parathion Hydrolysis by Pseudomonas diminuta-Reference-Cited by-同舟云学术

Plasmid Involvement in Parathion Hydrolysis by Pseudomonas diminuta

Published:1982-07 Issue:1 Volume:44 Page:246-249
ISSN:0099-2240
Container-title:Applied and Environmental Microbiology
language:en
Short-container-title:Appl Environ Microbiol

Author:

Serdar Cüneyt M.¹,Gibson David T.¹,Munnecke Douglas M.¹,Lancaster John H.¹

Affiliation:

1. Center for Applied Microbiology, Department of Microbiology, The University of Texas at Austin, Austin, Texas 78712 and Department of Botany and Microbiology, University of Oklahoma, Norman, Oklahoma 730192

Abstract

An organism identified as Pseudomonas diminuta was found to hydrolyze parathion. Cells grown for 48 h contained 3,400 U of parathion hydrolase activity per liter of broth. Expression of enzymatic activity was lost at a high frequency (9 to 12%) after treatment with mitomycin C. Hydrolase-negative derivatives were missing a plasmid present in the wild-type organism. The molecular mass of this plasmid (pCS1), as determined by electron microscopy, was about 44 × 10 6 daltons.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Link

https://journals.asm.org/doi/pdf/10.1128/aem.44.1.246-249.1982

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