Complete Genome Sequence of Dehalobacterium formicoaceticum Strain DMC, a Strictly Anaerobic Dichloromethane-Degrading Bacterium

Author:

Chen Gao12,Murdoch Robert W.1,Mack E. Erin3,Seger Edward S.4,Löffler Frank E.12567ORCID

Affiliation:

1. Center for Environmental Biotechnology, University of Tennessee, Knoxville, Tennessee, USA

2. Department of Civil and Environmental Engineering, University of Tennessee, Knoxville, Tennessee, USA

3. Corporate Remediation Group, E. I. DuPont de Nemours and Company, Newark, Delaware, USA

4. The Chemours Company, Wilmington, Delaware, USA

5. Department of Microbiology, University of Tennessee, Knoxville, Tennessee, USA

6. Department of Biosystems Engineering and Soil Science, University of Tennessee, Knoxville, Tennessee, USA

7. Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee, USA

Abstract

ABSTRACT Dehalobacterium formicoaceticum utilizes dichloromethane as the sole energy source in defined anoxic bicarbonate-buffered mineral salt medium. The products are formate, acetate, inorganic chloride, and biomass. The bacterium’s genome was sequenced using PacBio, assembled, and annotated. The complete genome consists of one 3.77-Mb circular chromosome harboring 3,935 predicted protein-encoding genes.

Funder

The Chemours Company

Publisher

American Society for Microbiology

Subject

Genetics,Molecular Biology

Reference23 articles.

1. Gribble GW . 2010. Naturally occurring organohalogen compounds—a comprehensive update, p 12–13. InProgress in the chemistry of organic natural products, vol 91. Springer, Vienna, Austria.

2. Bacterial Degradation of Dichloromethane

3. Dichloromethane dehalogenase of Hyphomicrobium sp. strain DM2

4. Bacterial growth with chlorinated methanes;Leisinger T;Environ Health Perspect,1995

5. Biodegradation of dichloromethane and its utilization as a growth substrate under methanogenic conditions

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