Complete Genome Sequence of the WHO International Standard for HIV-1 RNA Determined by Deep Sequencing

Author:

Gall Astrid1,Morris Clare2,Kellam Paul13,Berry Neil2

Affiliation:

1. Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge, United Kingdom

2. Division of Virology, National Institute for Biological Standards and Control, South Mimms, Potters Bar, United Kingdom

3. Research Department of Infection, Division of Infection and Immunity, University College London, London, United Kingdom

Abstract

ABSTRACT The World Health Organization (WHO) International Standard for HIV-1 RNA nucleic acid assays was characterized by complete genome deep sequencing analysis. The entire coding sequence and flanking long terminal repeats (LTRs), including minority species, were assigned subtype B. This information will aid the design, development, and evaluation of HIV-1 RNA amplification assays.

Publisher

American Society for Microbiology

Subject

Genetics,Molecular Biology

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