Affiliation:
1. Department of Microbiology, Seton Hall College of Medicine and Dentistry, Jersey City, New Jersey
Abstract
Cirillo, Vincent
P. (Seton Hall College of Medicine and Dentistry, Jersey City, N.J.). Mechanism of glucose transport across the yeast cell membrane. J. Bacteriol.
84:
485–491. 1962.—The kinetics of
d
-glucose and
l
-sorbose transport was studied in
Saccharomyces cerevisiae
inhibited with iodoacetic acid under nitrogen to prevent glucose metabolism.
d
-Glucose was found to compete with
l
-sorbose for a common membrane transport system with an apparent affinity greater than 25 times that of sorbose. A comparison of the net rate of glucose and sorbose transport at 50 and 500 m
m
external concentration showed that glucose transport is greater than that of sorbose from the lower concentration, but sorbose transport is greater than glucose at the higher concentration. This reversal of transport rate of two sugars with markedly different affinities is predicted by the membrane carrier theory. A further prediction of carrier theory was confirmed by the demonstration that the rate of glucose transport into fructose-loaded cells is greater than into unloaded cells.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
Reference23 articles.
1. Accumulation of trehalose and sucrose in relation to the metabolism of a-glucosides in yeast of defined genotype;AVIGAD A.;Biochim. et Biophys. Acta,1960
2. Transport of some monosaccharides into yeast cells;BURGER M., L.;Biochem. J.,1959
3. CIRILLO V. P. 1961a. The transport of nonfermentable sugars across the yeast cell membrane p. 343-351. In A. Kleinzeller and A. Kotyk [ed.] Membrane transport and metabolism. Academic Press Inc. New York.
4. The mechanism of sugar transport into the yeast cell. Trans. N.Y;CIRILLO V. P.;Acad. Sci. Ser. II,1961
5. Sugar transport in microorganisms;CIRILLO V. P.;Ann. Rev. Microbiol.,1961
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