Simultaneous Immunofluorescent Detection of Coentrapped Cells in Gel Beads-Reference-Cited by-同舟云学术

Simultaneous Immunofluorescent Detection of Coentrapped Cells in Gel Beads

Published:2000-05 Issue:5 Volume:66 Page:2216-2219
ISSN:0099-2240
Container-title:Applied and Environmental Microbiology
language:en
Short-container-title:Appl Environ Microbiol

Author:

Prioult Guenolee¹,Lacroix Christophe¹,Turcotte Carl¹,Fliss Ismaïl¹

Affiliation:

1. Dairy Research Centre STELA, Pavillon Paul Comtois, Université Laval, Quebec, Canada G1K 7P4

Abstract

ABSTRACT An immunofluorescent method involving double color labeling and confocal microscopy was reported to specifically detect lactic acid bacteria and probiotic cells coimmobilized in gels beads. The method described is rapid (4 h) and sensitive and may be useful for studying cell dynamics during mixed-culture starter production using immobilized cells in gel beads. Microscopic observations were perfectly correlated to cell counts obtained using a sandwich enzyme-linked immunosorbent assay.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Link

https://journals.asm.org/doi/pdf/10.1128/AEM.66.5.2216-2219.2000

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