Mitochondrial Citrate Transporters CtpA and YhmA Are Required for Extracellular Citric Acid Accumulation and Contribute to Cytosolic Acetyl Coenzyme A Generation in Aspergillus luchuensis mut. kawachii-Reference-Cited by-同舟云学术

Mitochondrial Citrate Transporters CtpA and YhmA Are Required for Extracellular Citric Acid Accumulation and Contribute to Cytosolic Acetyl Coenzyme A Generation in Aspergillus luchuensis mut. kawachii

Published:2019-04-15 Issue:8 Volume:85 Page:
ISSN:0099-2240
Container-title:Applied and Environmental Microbiology
language:en
Short-container-title:Appl Environ Microbiol

Author:

Kadooka Chihiro¹²,Izumitsu Kosuke³,Onoue Masahira⁴,Okutsu Kayu²,Yoshizaki Yumiko¹²,Takamine Kazunori¹²,Goto Masatoshi¹⁵,Tamaki Hisanori¹²,Futagami Taiki¹²^ORCID

Affiliation:

1. United Graduate School of Agricultural Sciences, Kagoshima University, Kagoshima, Japan

2. Education and Research Center for Fermentation Studies, Faculty of Agriculture, Kagoshima University, Kagoshima, Japan

3. School of Environmental Science, University of Shiga Prefecture, Hikone, Japan

4. Research Support Center, Institute for Research Promotion, Kagoshima University, Kagoshima, Japan

5. Faculty of Agriculture, Saga University, Saga, Japan

Abstract

Citrate transport is believed to play a significant role in citrate production by filamentous fungi; however, details of the process remain unclear. This study characterized two citrate transporters from Aspergillus luchuensis mut. kawachii . Biochemical and gene disruption analyses showed that CtpA and YhmA are mitochondrial citrate transporters required for normal hyphal growth, conidium formation, cytosolic acetyl-CoA synthesis, and citric acid production. The characteristics of fungal citrate transporters elucidated in this study will help expand our understanding of the citrate production mechanism and facilitate the development and optimization of industrial organic acid fermentation processes.

Funder

Sasakawa Scientific Research Grant

Grant-in-Aid for Scientific Research

JSPS Research Fellowship

Yonemori Seishin Ikuseikai

Institute for Fermentation, Osaka

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Link

https://journals.asm.org/doi/pdf/10.1128/AEM.03136-18

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