Production and Regeneration of Thiobacillus ferrooxidans Spheroplasts

Author:

Barros M. E. C.1,Rawlings D. E.1,Woods D. R.1

Affiliation:

1. Department of Microbiology, University of Cape Town, Rondebosch 7700, South Africa

Abstract

We investigated the production and regeneration of Thiobacillus ferrooxidans spheroplasts. Regeneration frequencies of up to 90% reversion were obtained. Attempts to transform T. ferrooxidans spheroplasts with a recombinant T. ferrooxidans plasmid were unsuccessful.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Reference21 articles.

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2. Mixotrophic growth of a Thiobacillusferrooxidans strain;Barros M. E. C.;Appl. Environ. Microbiol.,1984

3. Transfection of Enterobacteriaceae and its applications;Benzinger R.;Microbiol. Rev.,1978

4. Microbiological mining. Sci;Brierley C. L.;Am.,1982

5. Genetic recombination in fused spheroplasts of Providence alcalifaciens;Coetzee J. N.;J. Gen. Microbiol.,1979

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