Affiliation:
1. Institute of Microbiology, Shandong University, Jinan, People's Republic of China.
Abstract
The broad-host-range IncP plasmids RP4, R68.45, RP1::Tn501, and pUB307 were transferred to acidophilic, obligately chemolithotrophic Thiobacillus ferrooxidans from Escherichia coli by conjugation. A genetic marker of kanamycin resistance was expressed in T. ferrooxidans. Plasmid RP4 was transferred back to E. coli from T. ferrooxidans. The broad-host-range IncQ vector pJRD215 was mobilized to T. ferrooxidans with the aid of plasmid RP4 integrated in the chromosome of E. coli SM10. pJRD215 was stable, and all genetic markers (kanamycin/neomycin and streptomycin resistance) were expressed in T. ferrooxidans. By the use of suicide vector pSUP1011, transposon Tn5 was introduced into T. ferrooxidans. The influence of some factors on plasmid transfer from E. coli to T. ferrooxidans was investigated. Results showed that the physiological state of donor cells might be important to the mobilization of plasmids. The transfer of plasmids from E. coli to T. ferrooxidans occurred in the absence of energy sources for both donor and recipient.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
Reference37 articles.
1. Cloning and expression of the Thiobacillus ferrooxidans glutamine synthetase gene in Escherichia coli;Barros M. E. C.;J. Bacteriol.,1985
2. Production and regeneration of Thiobacillus ferrooxidans spheroplasts;Barros M. E. C.;Appl. Environ. Microbiol.,1985
3. Transposition of TnA does not generate deletions;Benett P. M.;Mol. Gen. Genet.,1977
4. Berg D. E. 1977. Insertion and excision of the transposable kanamycin resistance determinant Tn 5 p. 205-212. In A. I. Bukari J. A. Shapiro S. L. Adhya (ed.) DNA insertion elements plasmids and episomes. Cold Spring Harbor Laboratory Cold Spring Harbor N.Y.
5. The prokaryotic transposable element Tn 5;Berg D. E.;Bio/Technology,1983
Cited by
58 articles.
订阅此论文施引文献
订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献