Evaluation of the New NucliSENS EasyQ KPC Test for Rapid Detection of Klebsiella pneumoniae Carbapenemase Genes ( bla KPC )-Reference-Cited by-同舟云学术

Evaluation of the New NucliSENS EasyQ KPC Test for Rapid Detection of Klebsiella pneumoniae Carbapenemase Genes ( bla KPC )

Published:2012-08 Issue:8 Volume:50 Page:2783-2785
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Spanu Teresa¹,Fiori Barbara¹,D'Inzeo Tiziana¹,Canu Giulia¹,Campoli Serena¹,Giani Tommaso²,Palucci Ivana¹,Tumbarello Mario³,Sanguinetti Maurizio¹,Rossolini Gian Maria²⁴

Affiliation:

1. Institutes of Microbiology, Agostino Gemelli Hospital, Catholic University of the Sacred Heart, Rome, Italy

2. Section of Microbiology, Department of Biotechnologies, University of Siena

3. Infectious Diseases, Agostino Gemelli Hospital, Catholic University of the Sacred Heart, Rome, Italy

4. Clinical Microbiology and Virology Unit, Siena University Hospital, Siena, Italy

Abstract

ABSTRACT KPC-type carbapenemases are emerging in Klebsiella pneumoniae and other Gram-negative pathogens worldwide. Rapid and sensitive detection of these resistance determinants has become relevant to clinical management and infection control. We evaluated the bioMérieux EasyQ real-time PCR assay for bla KPC detection with 300 members of the Enterobacteriaceae , including 29 control strains producing known carbapenemases and 271 nonreplicate clinical isolates. The EasyQ assay correctly detected all of the 111 isolates harboring bla KPC genes, with no false positives, and results were available within 2 h.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.00284-12

Reference28 articles.

1. Application of Minimal Sequence Quality Values Prevents Misidentification of the bla SHV Type in Single Bacterial Isolates Carrying Different SHV Extended-Spectrum β-Lactamase Genes

2. Multicenter Evaluation of a New DNA Microarray for Rapid Detection of Clinically RelevantblaGenes from β-Lactam-Resistant Gram-Negative Bacteria

3. Detection of KPC Carbapenem-Hydrolyzing Enzymes in Enterobacter spp. from Brooklyn, New York

4. Multiplex Real-Time PCR Assay for Detection and Classification of Klebsiella pneumoniae Carbapenemase Gene ( bla KPC ) Variants

5. Development and Evaluation of a Real-Time PCR Assay for Detection of Klebsiella pneumoniae Carbapenemase Genes

Cited by 38 articles. 订阅此论文施引文献订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献

1. From Species to Genes: A New Diagnostic Paradigm;Antibiotics;2024-07-17

2. Trends in the Determination of Antimicrobial Resistance in Aquaculture and Fisheries;Handbook on Antimicrobial Resistance;2023

3. Trends in the Determination of Antimicrobial Resistance in Aquaculture and Fisheries;Handbook on Antimicrobial Resistance;2023

4. Recent Advances in Nanotechnology for the Management of Klebsiella pneumoniae–Related Infections;Biosensors;2022-12-10

5. Modern Tools for Rapid Diagnostics of Antimicrobial Resistance;Frontiers in Cellular and Infection Microbiology;2020-07-15