Application of Minimal Sequence Quality Values Prevents Misidentification of the bla SHV Type in Single Bacterial Isolates Carrying Different SHV Extended-Spectrum β-Lactamase Genes

Abstract

ABSTRACT Nucleotide sequencing is the standard molecular method for determination of the β-lactamase gene present in an isolate. Using minimal sequence quality values prevents misidentification of bla SHV genes, as illustrated by three strains of three different species that each contained two different bla SHV alleles, SHV-2 and SHV-12.