Affiliation:
1. Research Immunology Laboratory, Albert Einstein Medical Center, Philadelphia, Pennsylvania 19141
Abstract
A preparation of pure type A toxin of
Clostridium botulinum
was labeled with
131
I in the presence of chloramine-T and carrier-free isotope. The radioactive toxin (
131
I Tox) was used in a radioimmunoassay procedure similar to that of Berson and Yalow. Dilutions of antibody to the toxin, capable of binding 50% of the added
131
I Tox, were mixed with a sample of the labeled toxin and various concentrations of unlabeled toxin (‚Cold’ Tox). When concentration of Cold Tox were plotted against the ratio
131
I bound/
131
I not bound, a standard curve was established that could be used to estimate the concentration of Cold Tox in a test mixture. This assay was sensitive to as little as 100 mouse minimum lethal dose and was highly specific for the serological type of the toxin used.
Publisher
American Society for Microbiology
Subject
General Pharmacology, Toxicology and Pharmaceutics,General Immunology and Microbiology,General Biochemistry, Genetics and Molecular Biology,General Medicine
Reference7 articles.
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5. Chromatographic isolation of the crystalline toxin of Clostridium botulinum type A;DasGupta B. R.;Biochem. Biophys. Res. Commun.,1966
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