Affiliation:
1. Food and Drug Administration, Atlanta, Georgia 30309.
Abstract
Hybridomas synthesizing monoclonal antibodies (MAbs) against type F Clostridium botulinum toxin were developed. MAb from one stable hybridoma, hybridoma 223, consisted of kappa light chains and an immunoglobulin G subclass 2a heavy chain. This MAb was used in a double-sandwich enzyme-linked immunosorbent assay to detect type F toxin in foods, culture fluids, and purified toxin preparations. The sensitivity of the double-sandwich enzyme-linked immunosorbent assay was approximately 10 mouse lethal doses of toxin per ml of toxic fluid.
Publisher
American Society for Microbiology
Subject
Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology
Reference15 articles.
1. Radioimmunoassay for type A toxin of Clostridium botulinum;Boroff D. A.;Appl. Microbiol.,1973
2. Monoclonal antibody for the detection of Clostridium botulinum type A toxin;Ferreira J. L.;Mol. Cell. Probes,1987
3. Food and Drug Administration. 1984. Clostridium botulinum p. 18.01-18.10. In Bacteriological analytical manual for foods 6th ed. Association of Official Analytical Chemists Arlington Va.
4. Production of monoclonal antibodies against Clostridium botulinum type E derivative toxin;Kamata Y.;FEMS Microbiol. Lett.,1985
5. The use of monoclonal antibodies to analyze the structure of Clostridium botulinum type E derivative toxin;Kozaki S.;Infect. Immun.,1986
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