Affiliation:
1. Department of Veterinary Science, North Dakota State University, Fargo 58105.
Abstract
Here we have used the bovine herpesvirus type 1 (BHV-1) rabbit model together with in situ nucleic acid hybridization to identify and map viral RNA present in latently infected neurons. Radioactively labeled cloned HindIII fragments representing most of the BHV-1 genome (Cooper strain) were individually hybridized to sections of trigeminal ganglia taken from rabbits during acute and latent stages of infection. Whereas all viral genomic fragments hybridized to lytically infected tissue culture cells and to acutely infected ganglia, only HindIII fragment D (map units 0.734 to 0.842) hybridized to latently infected ganglionic neurons. Additional in situ hybridization experiments using subcloned fragments of HindIII-D further mapped the latency-related viral RNA to a 1.9-kilobase region (map units 0.734 to 0.748) of the viral genome. These results indicate that BHV-1 gene transcription is restricted during the latent phase of infection; further, they suggest that specific viral transcription may be involved in establishment or maintenance of latent BHV-1 infection.
Publisher
American Society for Microbiology
Subject
Virology,Insect Science,Immunology,Microbiology
Reference23 articles.
1. A rapid alkaline extraction procedure for screening recombinant plasmid DNA;Birnboim H. C.;Nucleic Acids Res.,1979
2. Detection of viral sequences of low reiteration frequency by in situ hybridization;Brahic M.;Proc. Natl. Acad. Sci. USA,1978
3. Human 0-globin pre-mRNA synthesized in vitro is accurately spliced in xenopus oocyte nuclei;Green M. R.;Cell,1983
4. Experimental latent and recrudescent bovine herpesvirus-1 infections in calves;Homan E. J.;Am. J. Vet. Res.,1983
5. Isolation of bovine herpesvirus-1 from trigeminal ganglia of clinically normal cattle;Homan E. J.;Am. J. Vet. Res.,1980