Simultaneous Detection and Differentiation between Wild-Type and Vaccine Measles Viruses by a Multiplex Real-Time Reverse Transcription-PCR Assay-Reference-Cited by-同舟云学术

Simultaneous Detection and Differentiation between Wild-Type and Vaccine Measles Viruses by a Multiplex Real-Time Reverse Transcription-PCR Assay

Published:2019-04 Issue:4 Volume:57 Page:
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Pabbaraju Kanti¹,Gill Kara¹,Wong Anita A.¹,Tipples Graham A.²³,Hiebert Joanne⁴,Severini Alberto⁴⁵,Fonseca Kevin¹⁶,Tellier Raymond¹⁷

Affiliation:

1. Provincial Laboratory for Public Health, Calgary, Alberta, Canada

2. Provincial Laboratory for Public Health, Edmonton, Alberta, Canada

3. Department of Medical Microbiology and Immunology, University of Alberta, Edmonton, Alberta, Canada

4. National Microbiology Laboratory, Public Health Agency of Canada, Winnipeg, Manitoba, Canada

5. Department of Medical Microbiology, Faculty of Health Sciences, University of Manitoba, Winnipeg, Manitoba, Canada

6. Department of Microbiology, Immunology and Infectious Diseases, University of Calgary, Calgary, Alberta, Canada

7. Department of Pathology and Laboratory Medicine, University of Calgary, Calgary, Alberta, Canada

Abstract

Measles is one of the most contagious viral respiratory infections and was declared to be eliminated from Canada in 1998; however, measles cases and outbreaks still occur every year through reintroduction from other parts of the world. Laboratory confirmation of measles virus (MV) RNA by real-time PCR provides a definitive diagnosis, and molecular analysis to determine the genotype is the only way to distinguish between wild-type and vaccine strains.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.01828-18

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