Strengths and Limitations of Commercial Tests for Hepatitis C Virus RNA Quantification

Author:

Morishima Chihiro12,Chung Minjun2,Ng Ka Wing2,Brambilla Donald J.3,Gretch David R.24

Affiliation:

1. Departments of Pediatrics

2. Laboratory Medicine

3. New England Research Institutes, Watertown, Massachusetts

4. Medicine, University of Washington School of Medicine, Seattle, Washington

Abstract

ABSTRACT The sensitivity of the Roche COBAS Monitor v.2.0 test was slightly better than that of the Bayer bDNA 3.0 test, but the Monitor test underquantified specimens by 2.5- to 10.6-fold even at relatively low hepatitis C virus RNA concentrations. Dilution prior to assay minimally decreased the reproducibility of the Monitor assay, leading to the recommendation for all specimens to be diluted 1:100 prior to testing by this assay.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

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