Affiliation:
1. The Pediatric Infectious Disease Unit
2. Primary Health Care Clinic Center, Dimona A
3. Primary Health Care Clinic Center, Ksefa, Israel
4. The Clinical Microbiology Laboratory, Soroka University Medical Center and the Faculty of Health Sciences, Ben-Gurion University of the Negev, Beer-Sheva
Abstract
ABSTRACT
Streptococcus pneumoniae
and
Haemophilus influenzae
carriage is a useful index for measuring the emergence of resistance and outcome in vaccination trials. We performed a study to determine which sampling site, nasopharynx (NP) or oropharynx (OP), yields the highest rate of
S. pneumoniae
and
H. influenzae
isolation at different ages. Both NP and OP cultures were obtained from 216 children aged <60 months and their mothers. The total
S. pneumoniae
carriage rate was 68% among children and 15% among mothers (
P
< 0.001). Using NP alone for the isolation of
S. pneumoniae
would have missed 2, 2, and 42% and using OP alone would have missed 77, 66, and 45% of
S. pneumoniae
in children aged 0 to 23 months, 24 to 59 months, and mothers, respectively. Using NP cultures alone for
H. influenzae
would have missed 23, 24, and 81% of the isolates, respectively. The respective figures for
H. influenzae
isolation from OP alone are 38, 29, and 9%. In children,
S. pneumoniae
was carried mainly in the NP while
H. influenzae
was equally carried in the NP and OP. In mothers,
S. pneumoniae
was carried equally in the NP and OP while
H. influenzae
was carried significantly more often in the OP. In children,
H. influenzae
colonization increased during illness, mainly in the NP. Culturing only one site significantly reduced the recovery of
H. influenzae
at all ages. NP cultures for
S. pneumoniae
detected close to 100% of isolates in children but only 58% of isolates in mothers.
Publisher
American Society for Microbiology
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