Use of Genome Selected Repeated Sequences Increases the Sensitivity of PCR Detection of Tropheryma whipplei-Reference-Cited by-同舟云学术

Use of Genome Selected Repeated Sequences Increases the Sensitivity of PCR Detection of Tropheryma whipplei

Published:2004-01 Issue:1 Volume:42 Page:401-403
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Fenollar Florence¹,Fournier Pierre-Edouard¹,Robert Catherine¹,Raoult Didier¹

Affiliation:

1. Unité des Rickettsies, CNRS UMR 6020, IFR 48, Faculté de médecine, Université de la Méditerranée, 13385 Marseille cedex 05, France

Abstract

ABSTRACT The availability of the Tropheryma whipplei genome offers the putative possibility of choosing logical DNA targets. We applied a PCR assay (targeting repeated sequences of T. whipplei ) to samples from patients with Whipple's disease and to those from members of a control group. When compared to the results seen with regular PCR, the sensitivity of repeat PCR was significantly enhanced ( P = 0.02) without alteration of its specificity.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.42.1.401-403.2004

Reference22 articles.

1. Altwegg, M., A. Fleisch-Marx, D. Goldenberger, S. Hailemariam, A. Schaffner, and R. Kissling. 1996. Spondylodiscitis caused by Tropheryma whippelii. Schweiz. Med. Wochenschr.126:1495-1499.

2. Amsler, L., P. Bauerfeind, C. Nigg, R. Maibach, R. Steffen, and M. Altwegg. 2003. Prevalence of Tropheryma whipplei DNA in patients with various gastrointestinal diseases and in healthy controls. Infection31:81-85.

3. Bentley, S. D., M. Maiwald, L. D. Murphy, M. J. Pallen, C. A. Yeats, L. G. Dover, H. T. Norbertczak, G. S. Besra, M. A. Quail, D. E. Harris, A. von Herbay, A. Goble, S. Rutter, R. Squares, S. Squares, B. G. Barrell, J. Parkhill, and D. A. Relman. 2003. Sequencing and analysis of the genome of the Whipple's disease bacterium Tropheryma whipplei. Lancet361:637-644.

4. rpoB Sequence Analysis of Cultured Tropheryma whippelii

5. Dutly, F., H. Hinrikson, T. Seidel, S. Morgenegg, M. Altwegg, and P. Bauerfeind. 2000. Tropheryma whippelii DNA in saliva of patients without Whipple's disease. Infection28:219-222.

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