Accuracy of Burkholderia pseudomallei Identification Using the API 20NE System and a Latex Agglutination Test

Author:

Amornchai Premjit1,Chierakul Wirongrong1,Wuthiekanun Vanaporn1,Mahakhunkijcharoen Yuvadee1,Phetsouvanh Rattanaphone2,Currie Bart J.3,Newton Paul N.24,van Vinh Chau Nguyen5,Wongratanacheewin Surasakdi6,Day Nicholas P. J.14,Peacock Sharon J.14

Affiliation:

1. Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand

2. Wellcome Trust-Mahosot Hospital-Oxford Tropical Medicine Research Collaboration, Mahosot Hospital, Vientiane, Lao PDR

3. Menzies School of Health Research, Charles Darwin University, Darwin, Northern Territory, Australia

4. Nuffield Department of Clinical Medicine, University of Oxford, Oxford, United Kingdom

5. Hospital for Tropical Diseases, Ho Chi Minh City, Vietnam

6. Melioidosis Research Center, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand

Abstract

ABSTRACT In an evaluation of the API 20NE for the identification of Burkholderia spp., 792/800 (99%) Burkholderia pseudomallei and 17/19 (89%) B. cepacia isolates were correctly identified but 10 B. mallei and 98 B. thailandensis isolates were not correctly identified. A latex agglutination test was positive for 796/800 (99.5%) B. pseudomallei isolates and negative for 120 other oxidase-positive gram-negative bacilli.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

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