Preliminary Evaluation of the API 20NE and RapID NF Plus Systems for Rapid Identification of Burkholderia pseudomallei and B. mallei

Author:

Glass Mindy B.1,Popovic Tanja1

Affiliation:

1. Meningitis and Special Pathogens Branch, Division of Bacterial and Mycotic Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia

Abstract

ABSTRACT We evaluated the API 20NE and the RapID NF Plus systems with 58 Burkholderia pseudomallei and 23 B. mallei strains for identification of these agents, but neither was reliable for confirmatory identification, with only 0 to 60% strains identified accurately. A greater diversity of strains in the system databases would be beneficial.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Reference13 articles.

1. Ashdown, L. R. 1979. Identification of Pseudomonas pseudomallei in the clinical laboratory. J. Clin. Pathol.32:500-504.

2. Centers for Disease Control and Prevention. 2000. Laboratory-acquired human glanders—Maryland, May 2000. Morb. Mortal. Wkly. Rep.49:532-535.

3. Dance, D. A., V. Wuthiekanun, P. Naigowit, and N. J. White. 1989. Identification of Pseudomonas pseudomallei in clinical practice: use of simple screening tests and API 20NE. J. Clin. Pathol.42:645-648.

4. Use of 16S rRNA Gene Sequencing for Rapid Identification and Differentiation of Burkholderia pseudomallei and B. mallei

5. Inglis, T. J., D. Chiang, G. S. Lee, and L. Chor-Kiang. 1998. Potential misidentification of Burkholderia pseudomallei by API 20NE. Pathology30:62-64.

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